Tal Gilboa

Tal Gilboa
Tal Gilboa
Tal Gilboa publishes paper in Elife
Harvard University

Paper abstract:

Extracellular vesicles (EVs) are released by all cells into biofluids such as plasma. The separation of EVs from highly abundant free proteins and similarly sized lipoproteins remains technically challenging. We developed a digital ELISA assay based on Single Molecule Array (Simoa) technology for ApoB-100, the protein component of several lipoproteins. Combining this ApoB-100 assay with previously developed Simoa assays for albumin and three tetraspanin proteins found on EVs (Ter-Ovanesyan, Norman et al., 2021), we were able to measure the separation of EVs from both lipoproteins and free proteins. These methods will enable applications where high-purity EVs are required to both understand EV biology and profile EVs for biomarker discovery.

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